Mycobacterium – NTM-Susceptibility
Conformément au Règlement de l’Ontario 671/92 de la Loi sur les services en français, les renseignements d’analyses de laboratoire liés à la présente page ne sont offerts qu’en anglais parce qu’ils sont de nature scientifique ou technique et destinés uniquement à l’usage des fournisseurs de soins de santé qualifiés et non aux membres du public.
Testing Indications
To request susceptibility testing for NTM, contact the Public Health laboratory for approval.
Specimen Requirements
| Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
Mycobacterium – NTM Susceptibility |
Viable (Non-tuberculosis mycobacterium) NTM culture |
N/A |
Solid or liquid mycobacterium culture medium |
Submission and Collection Notes
Approval for NTM drug susceptibility testing must be given prior to specimen submission.
Approval is based on meeting disease criteria:
- Sterile site isolation, bronchoalveolar lavage (BAL) isolation, bronch isolation, multiple specimens with growth (e.g. sputum)
- Treatment is planned or ongoing
- Susceptibilities are needed to plan or modify treatment
Susceptibility testing must be made within 3 months of culture identification.
Susceptibility testing is performed once per year per patient unless a different organism grows.
Susceptibility testing is not performed on M. gordonae and M. paragordonae isolates as these are generally considered not clinically significant.
Storage and Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Place specimen container in the biohazard bag and seal bag, and insert the completed requisition in the pocket on the outside of the sealed biohazard bag.
Special Instructions
Specimens should be submitted to PHO laboratory ASAP where possible.
Test Frequency and Turnaround Time (TAT)
Susceptibility testing for NTM is performed at the National Reference Centre for Mycobacteriology (NRCM), Winnipeg.
TAT for NTM susceptibility results from NRCM may take 2-6 weeks from the date that the culture is submitted to them. Results may take longer depending on the organism submitted for testing (e.g. slow grower).
Susceptibility testing for NTM is performed at the National Reference Centre for Mycobacteriology (NRCM), Winnipeg.
If testing is approved by the laboratory, a subculture will be prepared from the original isolate (original isolates are available for 3 months from the date of identification). Once adequate growth has been attained the subculture will be shipped to the National Reference Center for Mycobacteriology (NRCM), Winnipeg.
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