MRSA – Methicillin Resistant Staphylococcus aureus – Confirmation
Conformément au Règlement de l’Ontario 671/92 de la Loi sur les services en français, les renseignements d’analyses de laboratoire liés à la présente page ne sont offerts qu’en anglais parce qu’ils sont de nature scientifique ou technique et destinés uniquement à l’usage des fournisseurs de soins de santé qualifiés et non aux membres du public.
Specimen Requirements
| Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
MRSA – Methicillin Resistant Staphylococcus aureus confirmation |
Pure viable subculture of the organism on appropriate media (eg Blood Agar) or a swab of a pure subculture in Amies transport medium2,3 |
N/A |
Submission and Collection Notes
Complete all fields on the Reference Bacteriology Requisition, including:
- test Requested
- specimen source
- culture information
- presumptive identification
- clinical/epidemiology information
If a swab is received the TAT will be delayed by at least 24 hours.
Primary cultures are unacceptable; they should be processed in the originating lab and will be rejected
Limitations
Mixed or non-viable cultures will not be tested. The submitter will be contacted by telephone. A written report will be issued to indicate that the test has been rejected.
Mis-labelled or un-labelled specimens will not be tested. A report will be sent stating “Specimen received un-labelled” or “Requisition identification does not match the specimen identification information. Please resubmit.
Storage and Transport
Label the specimen with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number.Failure to provide this information may result in rejection or testing delay.
Place specimen in biohazard bag and seal. Specimen should be transferred to the laboratory within 48 hours of collection.
Special Instructions
Transport a fresh subculture to ensure viability on receipt.
Test Frequency and Turnaround Time (TAT)
Methicillin Resistant Staphylococcus aureus cultures are tested Monday - Friday.
Turnaround time is 3-5 days.
All aerobic bacterial isolates are identified/confirmed by the following methods:
- gram stain
- catalase
- MALDI-TOF
- phenotypic and/or molecular methods
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