Bacillus anthracis – Confirmation
Conformément au Règlement de l’Ontario 671/92 de la Loi sur les services en français, les renseignements d’analyses de laboratoire liés à la présente page ne sont offerts qu’en anglais parce qu’ils sont de nature scientifique ou technique et destinés uniquement à l’usage des fournisseurs de soins de santé qualifiés et non aux membres du public.
Testing Indications
Submitting laboratories must contact Public Health Ontario laboratory Customer Service Centre by telephone prior to submission of the isolate.
Specimen Requirements
| Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
Bacillus anthracis – confirmation |
Pure viable subculture of organism on Columbia sheep blood agar plate |
N/A |
Columbia sheep blood agar plate |
Submission and Collection Notes
Complete all fields of the Reference Bacteriology Requisition, including:
- test(s) requested (under other: rule out Bacillus anthracis)
- culture information (motility and haemolysis result)
- clinical/epidemiology
- contact information
Specimens received on either dry or gel-based swabs will not be accepted and will be cancelled.
Limitations
Culture must be viable. Use a fresh subculture and ship in conditions to ensure viability on receipt.
Storage and Transport
Label the specimen with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Special Instructions
Bacillus anthracis must be handled in accordance with the Canadian Biosafety Standards and shipped in accordance with the Transportation of Dangerous Goods Regulations.
Test Frequency and Turnaround Time (TAT)
Testing is performed daily (Monday to Friday) as received. Turnaround time for test results is up to 3 days from receipt by PHO laboratory.
Isolates that are positive for Bacillus anthracis by the Canadian Laboratory Response Network (CLRN) PCR assay are forwarded to the National Microbiology Laboratory (NML) for confirmatory testing. Turnaround time for test results is up to 3 days from receipt by NML.
STAT and Critical Samples Testing
Urgent testing is available upon request by calling the Customer Service Centre 416-235-6556 or 1-877-604-4567.
Bacillus anthracis is confirmed using Canadian Laboratory Response Network (CLRN)Real Time-PCR (Polymerase Chain Reaction) method.
Algorithm
Any isolate that is positive by the CLRN real-time PCR assay is considered as presumptively positive for Bacillus anthracis and is forwarded to the National Microbiology Laboratory (NML) for confirmatory testing.
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