Hepatitis B – Serology Test Ordering Instructions

Consistent with O. Reg. 671/92 of the French Language Services Act, test ordering and instructions on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.

All sections of the General Test Requisition must be completed by authorized health care providers for each specimen submitted. Testing may be delayed or cancelled if clinical information is not provided.

Refer to Hepatitis B Virus Serology Test Information Sheet for detailed testing indications, specimens, methods and reports information.

Requisition selection(s)	Tests Performed	Notes
Chronic Infection	HBsAg, HBcAb Total (IgG+IgM)
Acute Infection	HBsAg, HBcAb Total (IgG+IgM)	HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive
Immune Status	Anti-HBs
Pre-chemotherapy screening	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	For cancer patients prior to commencing systemic therapy (chemotherapy, targeted therapy, immunotherapy)
Chronic and Immunity	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	A reason for testing must be indicated under Testing Indication(s) / Criteria (e.g. Dialysis)
Acute and Immunity	HBsAg, HBcAb Total (IgG+IgM), Anti-HBs	A reason for testing must be indicated under Testing Indications(s) / Criteria (e.g. Dialysis) HBcIgM will be performed only if HBcAb Total (IgG+IgM) is reactive
Chronic Infection and Test(s) Requested: HBeAg, HBeAb	HBsAg HBcAb Total (IgG+IgM)	Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive
Acute Infection and Test(s) Requested: HBeAg, HBeAb	HBsAg HBcAb Total (IgG+IgM)	Testing for HBeAg and HBeAb will only be performed if HBsAg is reactive
Test(s) Requested: HBsAg, Anti-HBS	HBsAg Anti-HBS	Do not complete the Hepatitis Serology section. Enter the specific test(s) under Test(s) Requested. Under Testing Indication(s) / Criteria, specify “Hepatitis B Exposure” under Other
Test(s) Requested: HBeAg and HBeAb or HBcIgM	HBeAg/HBeAb or HBcIgM	Do not complete the Hepatitis Serology section. Enter the specific test(s) under Test(s) Requested

Requisition selection(s)

Tests Performed

Notes

Requisition Demonstration

Chronic Infection

HBsAg,

HBcAb Total (IgG+IgM)

Hep B-1 Chronic Infection